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: Cotija and Bola de Ocosingo are artisanal ripened cheeses produced in Mexico. Both are created with raw bovine milk from free of charge-grazing cows and with no starter cultures. Unlike lifestyle-based mostly strategies, molecular methods for pathogen detection in food items enable a shorter turnaround time, better detection specificity, and stand for a decreased microbiological threat for your analyst. Within the existing investigation, we analyzed 111 cheese samples (ninety five Cotija and 16 Bola de Ocosingo) by qPCR (TaqMan®) following an enrichment-society action distinct to each foodborne bacterium. The results confirmed that 100% of your samples were being free of DNA from Listeria monocytogenes, Brucella spp.

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This means that a adverse qPCR amplification end result wouldn't be as a result of existence of any inhibitor within the reaction combination or to the DNA currently being in compact fragments that will protect against getting amplicons of roughly 200 bp, as in the situation of your qPCR amplicons predicted for our primer–probe sets.

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